Kevin Wang, PhD

Title: Director, Center for Neuroproteomics and Biomarkers Research

Associate Director, Center for Traumatic Brain Injury Studies

 

Associate Professor of Psychiatry and Neuroscience
Email: kwang@psychiatry.ufl.edu

Contact information:
Center for Traumatic Brain Injury Studies,
Evelyn F. & William L. McKnight Brain Institute of the University of Florida
PO Box 100256
Gainesville, FL 32610
Ph: 352-294-0031
Email: kwang@psychiatry.ufl.edu

Areas of Interest:
CNS injury, proteases, proteomics, calcium-binding proteins, biomarkers.

Education:
Ph.D. (Pharmaceutical Sciences)
University of British Columbia, Vancouver, Canada.
B.Sc. (Honors)-with Distinction Biochemistry (Major), Biomedical Science (Minor)
University of Guelph, Guelph, Canada

Awards:
5/1998 Parke-Davis R&D 1997 Managerial Award
4/1984 Silver Medal (Academic Excellence), Chemical Institute of Canada

Grants:
PI NINDS-NIH, R01 NS049175-01 A1, "Biochemical basis of axonal & myelin injury in head trauma" 2005-2009

PI, Daimonion Diagnostics LLC, "Biomarker Characterization for traumatic brain injury" 2005-2007

PI, Florida Alzheimer's Center & Research Institute, "A biochemical linkage between traumatic brain injury and alzheimer's disease" 2004-2006

Co-PI, DoD , DAMD17-03-1-0066, "Biochemical markers for brain injury: An integrated proteomics-based approach" 2003-2007

PI, University of Florida Research Opportunity Fund, "Developing novel protease-targeted PET-visible "Inhibitor-ligans" to image neuronal cell damage following traumatic brain injury and methamphetamine exposure in live animals" 2003-2004

Co-PI, DoD, DAMD17-01-1-0765, "Common mechanisms of neuronal cell death after exposure to diverse environmental insults: Implications for treatment" 2001-2005

Co-PI: NIH, R01, NS#39091-02, Proteolytic Mechanisms of TBI 2001-2004

Co-PI: NIH R01, NS #38105-01, Calpain-Calpastastin Modulation of TBI 2000-2003

PAPERS & BOOK CHAPTERS
-1. Wang, K.K.W., Villalobo, A. and Roufogalis, B.D. (1988) Activation of the Ca2+-ATPase of human erythrocyte membrane by an endogenous Ca2+-dependent neutral protease. Arch. Biochem. Biophys. 260, 696-704. [PDF]

-2. Olorunsogo, O.O., Villalobo, A., Wang, K.K.W. and Roufogalis, B.D. (1988) The effect of calmodulin on the interaction of carbodiimides with the purified human erythrocyte (Ca2+ +Mg2+)-ATPase. Biochem. Biophys. Acta 945, 33-40. [PDF]

-3. Wang, K.K.W., Roufogalis, B.D. and Villalobo, A. (1988) Further characterization of the calpain-mediated proteolysis of the human erythrocyte plasma membrane Ca2+ ATPase. Arch. Biochem. Biophys. 267, 317-327. [PDF]

-4. Wang, K.K.W., Roufogalis, B.D. and Villalobo, A. (1989) Calpain I activated Ca2+ transport by the human erythrocyte plasma membrane calcium pump In: Calcium Binding Proteins in Normal and Transformed Cells (Lawson, D.E.M. and Pochet, R., eds.) Plenum Publishing Co., pp. 171-176.

-5. Wang, K.K.W., Villalobo, A. and Roufogalis, B.D. (1989) Calmodulin-binding proteins as calpain substrates. (review) Biochem. J. 262, 693-706.

-6. Wang, K.K.W., Roufogalis, B.D. and Villalobo, A. (1989) Characterization of the fragmented forms of calcineurin produced by calpain I. Biochem. Cell Biol. 67, 703-711. [PDF]

-7. Wang, K.K.W., Roufogalis, B.D. and Villalobo, A. (1989) Calpain I activates Ca2+ transport by the reconstituted erythrocyte Ca2+ pump. J. Membrane Biol. 112, 233-245. [PDF]

-8. Wang, K.K.W. (1990) Developing selective inhibitors of calpain. (review) Trends Pharmacol. Sc. 11, 139-142. [PDF]

-9. Roufogalis, B.D., Brzuszczak, I., Xu, Y.-H., Conigrave, A.D., Machan, C.L. and Wang, K.K.W. (1990) Persistent Ca2+ -induced activation of erythrocyte membrane Ca2+-ATPase unrelated to calpain proteolysis. Arch. Biochem. Biophys. 279, 78-86. [PDF]

-10. Kuo, T.H., Wang, K.K.W., Carlock, L., Diglio, C. and Tsang, W. (1991) Phorbol ester induces both the gene expression and phosphorylation of the plasma membrane Ca2+ pump. J. Biol. Chem. 266, 2520-2525.

-11. Wang, K.K.W., Wright, L.C., Machan, C.L., Allen, B.G., Conigrave, A.D. and Roufogalis, B.D. (1991) Protein kinase C phosphorylates the carboxyl-terminal of the human erythrocyte plasma membrane Ca2+-ATPase. J. Biol. Chem. 266, 9078-9085.

-12. Wang, K.K.W., Du, Y.-S., Diglio, C., Tsang, W. and Kuo, T.H. (1991) Hormone-induced phosphorylation of the plasma membrane calcium pump in cultured aortic endothelial cells. Arch. Biochem. Biophys. 289, 103-108. [PDF]

-13. Wang, K.K.W., Kuo, T.H. and Roufogalis, B.D. (1991) Structure-function relationship of the plasma membrane Ca2+-ATPase revealed by V8 protease treatment. Biochem. J., 279, 537-544.

-14. Wang, K.K.W., Villalobo, A. and Roufogalis (1992) The plasma membrane calcium pump: a multiregulated transporter. (invited review) Trends Cell Biol. 2, 46-52.

-15. Kuo, T.H., Tsang, W., Wang, K.K.W. and Carlock, L. (1992) Simultaneous reduction of the sarcolemmal and SR calcium ATPase activities and gene expression in cardiomyopathic hamster. Biochim. Biophys. Acta 1138, 343-349. [PDF]

-16. Gilchrist, J.S.C., Wang, K.K.W., Katz, S., Belcastro, A.N. (1993) Calcium activated neutral protease effects upon skeletal muscle sarcoplasmic reticulum protein structure and calcium release. J. Biol. Chem. 267, 20857-20865.

-17. Buroker Kilgore, M. and Wang, K.K.W. (1993) A Coomassie brilliant blue G250-based colorimetric assay for measuring activity of calpain and other proteases. Anal. Biochem. 208, 387-392.

-18. Elivira, M., Diez, J.A, Wang, K.K.W., Villalobo, A. (1993) Phosphorylation of connexin-32 by protein kinase C prevents its proteolysis by calpain I. J. Biol. Chem. 268, 14294-14300.

-19. Roufogalis, B.D. and Wang, K.K.W. (1993) The Plasma Membrane Calcium Pump (invited review) Today's Life Science 5, (2) 28-34; (3) 24-30. [PDF 1] [PDF 2]

-20. Khan, M.T., Wang, K.K.W., Villalobo, A. and Roufogalis, B.D. (1994) Characterization of a novel high molecular mass protein with peptidase activity purified from the human erythrocyte membrane by calmodulin affinity chromatography. J. Biol. Chem. 269, 10016-10021. [PDF]

-21. Wang, K.K.W. and Yuen, P.w. (1994) (invited review) Calpain inhibition: an overview of its therapeutic potentials. Trends Pharmacol. Sci. 15, 412-419. [PDF]

-22. Elvira, M., Wang, K.K.W. and Villalobo, A. (1994) Phosphorylated and non-phosphorylated connexin-32 molecules in gap junction plaques are protected against calpain proteolysis after protein kinase C-phosphorylation. Biochem. Soc. Trans. 22, 793-796. [PDF]

-23. De Jongh K., Colvin, A.A., Wang, K.K.W. and Catterall, W.A. (1994) Differential Proteolysis of the full-length form of the L-type calcium channel alpha 1 subunit by calpain. J. Neurochem. 63, 1558-1564. [PDF]

-24. Hajimohammadreza, I., Probert, A.W., Coughenour, L.L., Borosky, S.A., Boxer, P.A., Marcoux, F.W., Wang, K.K.W. (1995) Inhibition of Calmodulin-Dependent Protein Kinase-II Attenuates Excitotoxic Amino Acid Mediated Neuronal Death. J. Neurosci. 15, 4093-4101. [PDF]

-25. Raser, K.J., Posner, A. and Wang, K.K.W. (1995) Casein zymography: a method to study µ -calpain, m-calpain and their inhibitory agents. Arch. Biochem. Biophys. 319, 211-216.[PDF]

-26. Posner, A., Raser, K. and Wang, K.K.W. (1995) Aurintricarboxylic acid is an inhibitor of µ -calpain and m-calpain. Biochem. Mol. Biol. Int. 36, 291-299. [PDF]

-27. Watson, P.H., Mortimer, S.T., Wang, K.K.W., Croall, D.E. and Hanley, D.A. (1995) Calcium activated proteases in the bovine parathyroid gland: potential role in degradation of parathyroid hormone to peptide fragments. J. Molecular Endocrinology. 15, 61-71. [PDF]

-28. Wang, K.K.W. (1996) Intracellular Ca2+-binding proteins.(review) In Principles of Medical Biology (Eds. E.E. Bittar and N. Bittar), JAI Press Inc., Greenwich, Connecticut, pp. 253-270. [PDF]

29. Raser, K.J., Buroker-Kilgore, M. and Wang, K.K.W. (1996) Inactivation of calpain by terbium ion through binding interaction. Biochim. Biophys. Acta 1292, 9-14. [PDF]

-30. Wang, K.K.W., Posner, A. and Hajimohammadreza, I. (1996) Improved method of total protein extraction from cultured cells for use in protein electrophoresis and Western blotting. Biotechniques, 20, 662-668. [PDF]

-31. Wang, K.K.W., Posner, A., Raser, K.J., Buroker-Kilgore, M., Probert, A.W., Marcoux, F.W., Hays, S.J., Lunney, E. and Hajimohammadreza, I., Yuen, P.-w. (1996) Alpha-mercaptoacrylic acid derivatives as novel selective calpain inhibitors. In: Intracellular Protein Catabolism (Ed. K. Suzuki, J.S. Bond), Plenum Publishing. 95-102.

-32. Wang, K.K.W., Nath, R., Posner, A., Raser, K.J., Buroker-Kilgore, M., Ye, Q., Takano, E., Hatanaka, M., Maki, M., Marcoux, F.W., Caner, H., Collins, J.L., Fergus, A., Lee, K.S., Lunney, E.A., Hays, S.J. and Yuen, P.-w. (1996) Alpha-Mercaptoacrylic Acid Derivatives Are Selective Non-peptide and Calcium-Binding Domain-targeting Calpain Inhibitors. Proc. Natl. Acad. Sci. 93, 6687-6692.[PDF]

-33. Wang, K.K.W., Hajimohammadreza, I., Raser, K.J. and Nath, R. (1996) Maitotoxin induces calpain activation in SH-SY5Y neuroblastoma cells and cerebrocortical cultures. Arch. Biochem. Biophys. 331, 208-214.[PDF]

-34. Hell, J.W., Westenbroek, R.E., Breeze, L.J., Wang, K.K.W., Chavkin, C. and Catterall, W.A. (1996) NMDA receptor-induced proteolytic conversion of postsynaptic class C L-type calcium channels in hippocampal neurons. Proc. Natl. Acad. Sci. 93, 3362-3367.

-35. Vartanian, M.G., Cordon, J.J., Kupina, N.C., Schielke, G., Posner, A., Raser, K.J., Wang, K.K.W. and Taylor, C.P. (1996) Phenytoin pretreatment prevents hypoxic-ischemic brain damage in neonatal rats, Develop. Brain Res. 95, 169-175. [PDF]

-36. Edelstein, C.L., Yaqoob, M.M., Akhunaizi, A.M., Gengaro, P.E., Nemenoff, R.A., Wang, K.K.W., Schrier, R.W. (1996) Modulation of hypoxia-induced calpain activity in rat renal proximal tubules. Kidney Int. 50, 1150-1157. [PDF]

-37. Yuen, P.-w. and Wang, K.K.W. (1996) Therapeutic potential of calpain inhibitors in neurodegenerative disorders. Exp. Opin. Invest. Drugs 5, 1291-1304.[PDF]

-38. Nath, R., Raser, K.J., Stafford, D., Hajimohammadreza, I., Posner, A., Allen, H., Talanian, R.V., Yuen, P.-W., Gilbertsen, R.B. and Wang, K.K.W. (1996) Nonerythroid alpha-Spectrin Breakdown by Calpain and ICE-like protease(s) in Apoptotic Cells: Contributory Roles of both protease families in Neuronal Apoptosis. Biochem. J. 319, 683-690.[PDF]

-39. Nath, R., Raser, K.J., McGinnis, K., Stafford, D. and Wang, K.K.W. (1996) Effects of Calpain and ICE-like Protease Inhibitors on Neuronal Apoptosis. NeuroReport 8, 249-256.

-40. Wang, K.K.W. and Yuen, P.-w. (1997) Development and Therapeutic Potential of Calpain Inhibitors. Adv. Pharmacol. 37, 117-152.[PDF]

-41. Gilbertsen, R.B., Posmantur, R., Nath, R., Wang, K.K.W. (1997) Apoptotic death induced in MOLT-4 T-lymphoblasts by purine nucleoside phosphorylase inhibition. Inflamm. Res. 46, S151-S152.

-42. Posmantur, R., McGinnis, Nadimpalli, R., Gilbertsen, R. and Wang, K.K.W. (1997) Characterization of CPP32-like protease activity following apoptotic challenge in SH-SY5Y neuroblastoma cells. J. Neurochem. 68, 2328-2337.

-43. Hajimohammadreza, I., Nath, R., Raser, K.J., Nadimpalli, R. and Wang, K.K.W. (1997) Proteolysis of Neuronal nitric oxide synthase and calmodulin-dependent protein kinase IIalpha undergo neurotoxin-induced proteolysis. J. Neurochem. 69, 1006-1013.[PDF]

-44. Lin, G.-d., Chattopadhyay, D., Maki, M., Wang, K.K.W., Carson, M., Takana, E., Hatanaka, M., DeLucas, L.J., Narayana, S.V.L. (1997) Crystal structure of calcium bound domain VI of calpain at 1.9A resolution and its role in enzyme assembly and inhibitor binding. Nature Structure Biology 4, 539-547.

-45. Posmantur, R., Wang, K.K.W., Nath, R., Gilbertsen, R., (1997) a purine nucleoside phosphorylase (PNP) inhibitor induces apoposis via caspase-3 like protease activity in Molt-4 cells. Immunopharmacology 37, 231-244. [PDF]

-46. Nath, R., Hajimohammadreza, I. and Wang, K.K.W. (1997) Thapsigargin induces apoptosis in SH-SY5Y neuroblastoma acells and cerebrocortical cultures. Biochem. Mol. Biol. Int. 43, 197-205.

-47. Waters, S.L., Sarang, S.S., Wang, K.K.W. and Schnellmann, R.G. (1998) Calpains mediate calcium and chloride influx during the late phase of cell injury. J. Pharmacol. Exp. Ther. 283, 1177-1184.

-48. Zhao, X., Posmantur, R., Kampfl, A., Liu, S.J., Wang, K.K.W., Newcomb, J.K., Pike, B., Clifton, G.L. and Hayes, R.L. (1998) Subcellular localization and duration of µ -calpain and m-calpainm activity following traumatic brain injury in the rat: A casein zymography study. J. Cereb. Blood Flow Metab., 18, 161-167. [PDF]

-49. Hays, S.J., Caprathe, B.W., Gilmore, J.L., Amin, N., Emmerlings, M.R., Michael, W., Nadimpalli, R., Nath, R., Stafford, D., Watson, D., Wang, K.K.W., Jaen, J.C. (1998) 2-Amino-4H-3,1-benzoxazin-4-ones as inhibitors of C1r serine protease. J. Med. Chem. 41, 1060-1067. [PDF]

-50. Yuen, P.-w. and Wang, K.K.W. (1998) Calpain Inhibitors: Novel Neuroprotectants and Potential Anticataractic Agents. Drug of Future, 23 741-749.[PDF]

-51. Nath, R., Probert, A., Jr., McGinnis, K.M., Wang, K.K.W. (1998) Evidence for Activation of Caspase-3-like Protease in Excitotoxins- and Hypoxia/hypoglycemia-Injured Neurons. J. Neurochem. 71, 186-195.[PDF]

-52. Wang, K.K.W., Posmantur, R., Nath, R., McGinnis, K., Whitton, M., Talanian, R.V., Glantz, S., Morrow, J. (1998) Simultaneous Degradation of alphaII- and BetaII-Spectrin by Caspase 3 (CPP32) in Apoptotic Cells. J. Biol. Chem. 273, 22490-22497.[PDF]

-53. Hayes, R.L., Wang, K.K.W., Kampfl, A., Posmantur, R.M., Newcomb, J.K. and Clifton, G.L. (1998) The potential contribution of proteases to Neuronal damage. Drug News Perspect. 11, 215-222. [PDF]

-54. Pike, B.R., Zhao, X., Newcomb, J.K., Wang, K.K.W., Posmantur, R.M., Hayes, R.L. (1998) Temporal Relationships between de novo protein synthesis, calpain and caspase 3-like protease activation, and DNA Fragmentation during apoptosis in sept-hippocampal cultures. J. Neurosci. Res. 52, 505-520. [PDF]

-55. Zhao, X., Newcomb, J.K., Posmantur, R.M., Wang, K.K.W., Pike, B.R. and Hayes, R.L. (1998) pH dependency of µ -calpain and m-calpain activity assayed by casein zymography following traumatic brain injury in the rat. Neurosci. Lett. 247, 53-57. [PDF]

-56. McGinnis, K.M., Whitton, M., Gnegy, M.E., Wang, K.K.W. (1998) Calmodulin-dependent protein kinase IV differentially cleaved by caspase-3 and calpain in SH-SY5Y human neuroblastoma cells. J. Biol. Chem. 273, 19993-20000.[PDF]

-57. Wang, K.K.W., Posmantur, R., Nadimappli, R., Nath, R., Mohan, Nixon, R. Talanian, R.V., Allen, H. (1998) Caspase-mediated Fragmentation of Calpain Inhibitor Protein Calpastatin during apoptosis. Arch. Biochem. Biophys. 356, 187-196.[PDF]

-58. Posmantur, R.M., Wang, K.K.W., Gilbertsen, R.B. (1998) Caspase-3 like activity is necessary for IL-2 release in Activated Jurkat T-cells. Exp. Cell Res. 244, 302-309.[PDF]

-59. Pike, B.R., Zhao, X., Newcomb, J.K., Posmantur, R.M., Wang, K.K.W., Hayes, R.L. (1998) Regional calpain and caspase-3 proteolysis of alpha-spectrin after traumatic brain injury. NeuroReport. 9, 2437-2442.[PDF]

-60. Plummer J.S., Cai, C., Hays, S.J., Gilmore, J.L., Emmerling, M.R., Michael, W., Narasimhan, L.S., Watson, M.D., Wang, K.K.W., Nath, R. and Jaen, J.C. (1999) Benzenesulfonamide derivatives of 2-substituted 4h-3,1-benzoxazin-4-ones and benzthiazin4-ones as inhibitors of complement c1r protease. Bioorg. Med. Chem. Lett. 9, 815-820. [PDF]

-61. Wang, K.K.W. (1999) Detection of proteolytic enzymes using protein substrates. In Proteolytic Enzymes -Tools and Targets (Springer Lab Manual) (Sterchi, E.E., Stocker, W. Eds.) Springer-Verlag, Heidelberg, Chapter 4, pp. 49-62. [PDF]

-62. Wang K.K.W. and Yuen, P.-w. (1999) Calpain Substrates, Assay Methods, regulation and Inhibitory agents. In Calpain: Pharmacology and Toxicology of a Calcium-dependent Cellular Protease (Eds. K. Wang, P. Yuen), Taylor and Francis, Chapter 5, pp 77-101.

-63. Wang, K.K.W., Yuen, P.-w., Lee, K.S. (1999) Calpain in excitotoxicity, cerebral ischemia and neuronal apoptosis In Calpain: Pharmacology and Toxicology of a Calcium-dependent Cellular Protease (Eds. K. Wang, P. Yuen), Taylor and Francis, Chapter 9, pp 179-209.

-64. McGinnis, K.M., Nath, R., Wang, K.K.W., Posmantur, R. (1999) Proteases and Proteolysis in Neuronal Degeneration. In “Recent Research Developments in Neurochemistry” (Pandalai, S.G. Ed.) , Research Signpost, India, Vol. 2, 207-216.

-65. McGinnis, K.M., Gnegy, M.E. and Wang, K.K.W. (1999) Endogenous Bax translocation in SH-SY5Y human neuroblastoma cells and cerebellar granule neurons undergoing apoptosis. J. Neurochem. 72: 1899-1906.[PDF]

-66. McGinnis, K.M., Wang, K.K.W., Gnegy, M.E. (1999) Alternations of extracellular calcium elicit selective modes of cell death and protease activation in SH-SY5Y human neuroblastoma cells. J. Neurochem. 72, 1853-1863.[PDF]

-67. Zhao, X., Pike, B.R., Newcomb, J.K., Wang, K.K.W., Posmantur, R.M. and Hayes, R.L. (1999) Maitotoxin induces calpain but not caspase-3 activation and necrotic cell death in primary septo-hippocampal cultures. Neurochem. Res. 24: 371-382. [PDF]

-68. McGinnis, K.M., Gnegy, M.E., Park, Y.H., Mukerjee, N., Wang, K.K.W. (1999) Pro-caspase-3 and poly(ADP)ribose polymerase (PARP) are calpain substrates. Biochem. Biophys. Res. Commun.263, 94-99.[PDF]

-69. Nath, R., Scott,, M., Nadimpalli, R., Gupta, R. and Wang, K.K.W. (2000) Activation of Apoptosis-linked Caspase(s) in NMDA-injured brains in Neonatal Rat. Neurochem. Int. 36, 119-126.[PDF]

-70. Wang, K.K.W. (1999) Caspases: A Drug Discovery Perspective. Curr.Opin. Drug Discovery Develop. 2, 519-527. [PDF]

-71. Wang, K.K.W. (2000) Calpain and Caspase: Can You Tell the Difference. Trends Neurosci. 23, 20-26.[PDF]

-72. Nath, R., Huggins, M., McGinnis, K., Nadimpalli, D., Wang, K.K.W. (2000) Development and characterization of antibodies specific to caspase-3-produced alpha Ii-spectrin 120 kDa breakdown product: Marker for neuronal apoptosis. Neurochem. Int. 36, 351-361.[PDF]

-73. Zhao, X., Newcomb, J.K., Pike, B.R., Wang, K.K.W., d’Avella, D. and Hayes, R.L. (2000) Novel characteristics of glutamate-induced cell death in primary septohishippocampal cultures: Relationship to calpain and caspase-3 protease activation. J. Cereb. Blood Flow Metab. 20 (3) 550-562.

-74. Buki A.; Okonkwo D.O; Wang K. K W; Povlishock John T (2000) Cytochrome c release and caspase activation in traumatic axonal injury. J. Neuroscience 20 (8), 2825-2834.[PDF]

-75. Mukerjee, N., McGinnis, K.M., Park, Y.H., Gnegy, M.E., Wang, K.K.W. (2000) Caspase-mediated proteolytic activation of calcineurin in thapsigargin-mediated apoptosis in SH-SY5Y neuroblastoma cells. Arch. Biochem. Biophys. 379 :337-343. [PDF]

-76. Nath, R., Megan Davis, Albert W. Probert, Nancy C. Kupina, Xiaodong Ren, Gerald P. Schielke and Wang, K.K.W, (2000) Processing of cdk5 activator p35 to its truncated form (p25) by calpain in acutely injured neuronal cells. Biochem. Biophys. Res. Commun. 274, 16-21.[PDF]

-77. Zimmerman, U-J P., Boring, L., Pak, J.H., Mukerjee, N. and Wang, K.K.W. (2000) The calpain small subunit gene is essential: its inactivation results in embryonic lethality. IUBMB Life. 2000 Jul;50(1):63-68.[PDF]

-78. Y.H., Y.H. Sun, Y. and Wang, K.K.W. (2001) Cloning and characterization of a truncated caspase-3 variant having anti-apoptosis properties. Biochem. Biophys. Res. Commun. 283, 762-769.[PDF]

-79. Zhao X, Bausano B, Pike BR, Newcomb-Fernandez JK, Wang KKW, Shohami E, Ringger NC, DeFord SM, Anderson DK, Hayes RL. (2001) TNF-a Stimulates Caspase-3 Activation and Apoptotic Cell Death in Primary Septo-Hippocampal Cultures. J. Neurosci. Res. 64:121-131. [PDF]

-80. McGinnis, K. Wang, K.K.W., Gnegy, M.E. (2001) Calcium/calmodulin-dependent protein kinase inhibition potentiates thapsigargin-mediated cell death in SH-SY5Y human neuroblastoma cells. Neurosci Lett. 301: 99-102.[PDF]

-81. Huang, Y.H. and Wang, K.K.W., (2001) Mammalian Calpain Family and its Entanglement with Human Disease. Molecular Medicine Today 7, 355-362.[PDF]

-82. Nath, R., McGinnis, K., Dutta, S., Shivers, B., Wang, K.K.W. (2001) Inhibition of p38 kinase mimics survival signal-linked protection against apoptosis in rat cerebellar granule neurons. Cell Mol. Biol. Lett. 6, 173-184. [PDF]

-83. Mukerjee, N., McGinnis, K.M., Gnegy, M.E., Wang, K.K.W. (2001) Caspase-mediated calcineurin activation contributes to IL-2 release during T-cell activation. Biochem. Biophys. Res. Commun. 285, 1192-1199.[PDF]

-84. Perez, A., Probert, A.W., Wang, K.K.W., Sharmeen, L. (2000) Evaluation of HIV-1 Tat induced neurotoxicity in rat cortical cell culture.J Neurovirol. 2001 Feb;7(1):1-10.

-85. Pike, B.R., Flint, J, Johnson, E., Glenn, C.C., Dutta, S., Wang, KKW and Hayes, R.L. (2001) Accumulation of Calpain-Cleaved Non-Erythroid ?II-Spectrin in Cerebrospinal Fluid after Traumatic Brain Injury in Rats. J. Neurochem. 78, 1297-1306.[PDF]

-86. Beer R, Franz G, Krajewski S, Pike BR, Hayes RL, Reed JC, Wang KK, Klimmer C, Schmutzhard E, Poewe W, Kampfl A.(2001) Temporal and spatial profile of caspase 8 expression and proteolysis after experimental traumatic brain injury.J Neurochem. 78(4):862-873. [PDF]

-87. Zhao X, Bausano B, Pike BR, Newcomb-Fernandez JK, Wang KK, Shohami E, Ringger NC, DeFord SM, Anderson DK, Hayes RL. (2001) TNF-alpha stimulates caspase-3 activation and apoptotic cell death in primary septo-hippocampal cultures. J Neurosci Res. 64(2):121-131.

-88. Newcomb-Fernandez, J.K., Zhao, X., Pike, B.R., Wang, K.K.W., Kampfl, A., Beer, R., Deford, SM. and Hayes, R.L. (2002) Concurrent assessment of calpain and caspase-3 activation after oxygen-glucose deprivation in primary septo-hippocampal cultures. J. Cereb. Blood Flow Metab. 21, 1281-1294. [PDF]

-89. Kupina, N.C., Nath, R., Bernath, E.E. Inoue,J. Azuma, M. Yuen, P.-w. Wang K.K.W., and D. Hall, E.D. (2001) The Novel Calpain Inhibitor SJA6017 Improves Functional Recovery After Delayed Administration in a Mouse Model of Diffuse Head Injury. J. Neurtrauma. 18, 1229-1240.[PDF]

-90. Wang, K.K.W. (2002) Assaying proteases in cellular environments In: Current Protocols in Protein Science (Ed. J.E. Coligan, B. Dunn, H.L. Ploegh and D.W. Speicher) John Wilkey & Sons, Inc. New York. p.12. 21.1-21.12.14. [PDF]

-91. Wang, K.K.W. (2002) Therapeutic approaches with protease inhibitors in neurodegenerative and neurological diseases In: Role of Protease in the Pathology of Neurodegenerative Disease (N. Banik, A. Lajtha and Smith eds.), Kluwer Academic/Plenum Publishers, New York, Chapter 13.

-92. Wang, K.K.W. (2002) Calpain and caspase in ischemic and traumatic brain injury. In New Concepts in Cerebral Ischemia. (Ed. Rick Lin) CRC press, Chapter 9 , pp. 181-200. [PDF]

-93. Bian, F., Nath, R., Vergara, G., Booher, R., Lipinski, W.J., Callahan, M.J., Pack, A., Sobocinski, G., Wang, K.K.W., Walker, L.C. (2002) Tau hyperphosphorylation, axonal abnormalities and motor dysfunction in p25 protein–overexpressing transgenic mice. J. Com. Neurol. 446:257-266. [PDF]

-94. Dutta, S. Chiu, Y.C. Probert, A.W., Wang, K.K.W. (2002) Selective release of calpain produced alphaII-spectrin (alpha-fodrin) breakdown products from acute neuronal cell death. Biol. Chem. May;383(5):785-791.[PDF]

-95. Huang, Y., Tan, M., Gosink, M., Wang, K.K.W. and Sun, Y. (2002) Histone Deacetylase 5 is not a p53 target gene, but its overexpression inhibits tumor cell growth and induces apoptosis. Cancer Res. 62, 2913-2922.[PDF]

-96. Tolentino,P.J. Michelle, S., DeFord, Lucia Notterpek, L.,Glenn,C.C. Pike, P.R. Kevin K. Wang, K.K.W. and Hayes, R.L. (2002) Upregulation of tissue-type transglutaminase after traumatic brain injury. J Neurochem. 80:579-588.[PDF]

-97. Franz G, Beer R, Intemann D, Krajewski S, Reed JC, Engelhardt K, Pike BR, Hayes RL, Wang KK, Schmutzhard E, Kampfl A. (2002) Temporal and spatial profile of bid cleavage after experimental traumatic brain injury. J Cereb Blood Flow Metab. 22:951-958.

-98. Park, Y.H., Kantor, L., Wang, K.K.W., Gnegy, M.E. (2002) Repeated intermittent treatment with amphetamine induces neurite outgrowth in rat pheochromocytoma cells (PC12 cells). Brain Research 951, 43-52.[PDF]

-99. Kantor L, Park YH, Wang KK, Gnegy M. (2002) Enhanced amphetamine-mediated dopamine release develops in PC12 cells after repeated amphetamine treatment. Eur J Pharmacol. 2002 Sep 6;451(1):27-35.[PDF]

-100. Wang, K.K.W. (2003) Apoptosis in Neurodegeneration In: Handbook of Experimental Pharmacology; Volume - “CNS Neuroprotection” (F.W. Marcoux; D.W. Choi, Eds.) Springer-Verlag, Berlin, pp 37-64.

-101. McGinnis, K.M., Gnegy, M.E. and Wang, K.K.W. (2003) Cytochrome C translocation does not lead to caspase activation in maitotoxin-treated SH-SY5Y human neuroblastoma cells. Neurochem. Int 42(6):517-523.[PDF]

-102. Pike BR, Flint J, Dave, J.R., Lu, , X.-C. M, Wang KKW, Tortella, F.C., Hayes RL. (2003) Accumulation of calpain and caspase-3 proteolytic fragments of brain-derived aII-spectrin in CSF after middle cerebral artery occlusion in rats. J. Cereb. Blood Flow Metab. 24(1): 98-106.[PDF]

-103. Inoue J, Nakamura M, Cui YS, Sakai Y, Sakai O, Hill JR, Wang KKW, Yuen PW. (2003) Structure-activity relationship study and drug profile of N-(4-fluorophenylsulfonyl)-L-valyl-L-leucinal (SJA6017) as a potent calpain inhibitor. J Med Chem. 2003 Feb 27;46(5):868-71.[PDF]

-104. Park YH, Kantor L, Guptaroy B, Zhang M, Wang KK, Gnegy ME. (2003) Repeated amphetamine treatment induces neurite outgrowth and enhanced amphetamine-stimulated dopamine release in rat pheochromocytoma cells (PC12 cells) via a protein kinase C- and mitogen activated protein kinase-dependent mechanism.J Neurochem. 2003 Dec;87(6):1546-57.[PDF]

-105 Todd B, Moore D, Deivanayagam CC, Lin GD, Chattopadhyay D, Maki M, Wang KKW, Narayana SV. (2003) A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor. J Mol Biol. 2003 Apr 18;328(1):131-46.

-106. Pineda, J.A., Wang, K.K.W., Hayes, R.L. (2004) Biomarkers of Proteolytic Damage Following Traumatic Brain Injury. Brain Pathology, 14, 202-209.[PDF]

-107. Larner, S.F., McKinsey, D.M., Torres, M., Pike, M. Hayes, R.L. and Wang, KKW. (2004) Upregulation of caspase-12 after traumatic brain injury in rats. J. Neurochem. 88(1): 78-90.[PDF]

-108. Ellis, R.L., , Earnhardt, J.N., Hayes, R.L., Kevin K.W. Wang, K.K.W. and Anderson, D.K. (2004) Cathepsin B mRNA and protein expression following contusion spinal cord injury in rats. J. Neurochem. 88(3): 689-697. [PDF]

-109. Wang, K.K.W., Ottens, A., Haskins, W.E., Liu, M.C., Denslow, N., Chen,S.S., Hayes, R.L. (2004) Neuroproteomic studies of traumatic brain injury International Review of Neurobiology, 61, 215-240.(Review)

-110. Ringger, N.C., Tolentino, P.J., McKinsey, D.M., Pike, B.R., Wang, K.K.W. and Hayes, R.L. (2004) Effects of injury severity on regional and temporal mRNA expression levels of calpains and caspases after TBI. J. Neurotrauma, 21, 829-841.[PDF]

-111. Johnson EA, Svetlov SI, Pike BR, Tolentino PJ, Shaw G, Wang KK, Hayes RL, Pineda JA. (2004) Cell-specific upregulation of survivin after experimental traumatic brain injury in rats. J Neurotrauma. 2004 Sep;21(9):1183-95.

-112. Waghray, A., Wang, D., McKinsey, D.M., Hayes, R.L. and Wang, K.K.W. (2004) Molecular cloning and characterization of rat and human calpain-5. Biochem. Biophys. Res. Commun. 324, 46-51.[PDF]

-113. Tolentino PJ, Waghray A, Wang KK, Hayes RL. (2004) Increased expression of tissue-type transglutaminase following middle cerebral artery occlusion in rats. J Neurochem. 2004 Jun;89(5):1301-7.[PDF]

-114. Chen, SS, WE Haskins, Z Zhang, N Denslow, RL Hayes, KKW Wang and AK Ottens (2005) Bioinformatics for Traumatic Brain Injury: Proteomic Data Mining In "Data Mining in Biomedicine" (Ed. Panos Pardalos et al.) in press (Review).

-115. Svetlov, SI, TN Ignatova, KKW Wang, RL Hayes, VG Kukekov (2004) Growth and clonal expansion of neural stem/progenitor cells by lysophosphatidic acid (LPA) in mouse neurospheres. J. Hematotherapy & Stem Cell Research. 13:685-693.

-116. Johnson EA, SI Svetlov, BR Pike, PJ Tolentino, G Shaw, KKW Wang, RL Hayes, JA Pineda. (2004) Cell-specific upregulation of survivin after experimental traumatic brain injury in rats. J Neurotrauma 21:1183-95.

-117. Ellis, RL, W O’Steen, RL Hayes, KKW Wang, D Anderson (2005). Cellular localization and enzymatic activity of cathepsin B after spinal cord injury in the rat. Experimental Neurology 193:19-28.

-118. Ringger, NC, BE O’Steen, JG Brabham, X Silver, J Pineda, KKW Wang, and RL Hayes (2005) A novel marker for traumatic brain injury: CSF alphaII-spectrin breakdown product levels. J. Neurotrauma 21: 1443–1456.

-119. Haskins, WE, FH Kobeissy; RA Wolper, AK Ottens, JW Kitlen, SH McClung, BE O'Steen, MM Chow, JA Pineda, ND Denslow, RL Hayes, and KKW Wang (2005) Rapid discovery of putative protein biomarkers of traumatic brain injury by SDS-PAGE-capillary liquid chromatography-tandem mass spectrometry, J. Neurotrauma. 22:629-644. [PDF]

-120. Johnson, EA, S Svetlov, KKW Wang, RL Hayes, and JA Pineda (2005). “Cell-specific DNA fragmentation may bBe attenuated by a survivin-dependent mechanism following traumatic brain injury in rats”. Exp. Brain Res, 21:1183-1195.

-121. Larner, SF, DM McKinsey, RLHayes, and KKW Wang (2005) Caspase-7: Increased Expression and Activation Following Traumatic Brain Injury in Rats. J. Neurochem. 94:97-108.

-122. Bernath, E, MC Liu, RL Hayes, and KKW Wang (2005) Elevation of Cytoskeletal Protein Breakdown in Aged Wistar Rat. Brains. Neurobiol of Aging, epub. [PDF]

-123. Ottens AK, Kobeissy FH, Wolper RA, Haskins WE, Hayes RL, Denslow ND, Wang KKW. (2005) A Multidimensional Differential Proteomic Platform Using Dual Phase Ion-Exchange Chromatography - Polyacrylamide Gel Electrophoresis / Reverse Phase Liquid Chromatography Tandem Mass Spectrometry. Anal Chem, 77, 4836-4845. [PDF]

-124. Wang KKW, Ottens AK, Liu MC, Lewis SB, Meegan C, Oli MW, Tortella FC, Hayes RL. (2005) Proteomic identification of biomarkers of traumatic brain injury. Expert Rev Proteomics. (Invited Review) 2:603-614. [PDF]

-125. Kobeissy FH, Demetree JM, Zhang Z, Ottens AK, Warrens M, Wang KKW, Gold MS. (2005) A review of club drug use and abuse: Effects of MDMA, Methamphetamine, and CHB on Memory. J. Law Forum, 5:115-125. [PDF]

-126. Chen S, Haskins WE, Zhang Z, Hayes RL, Denslow ND, Wang KKW, Ottens AK. Bioinformatics for Traumatic Brain Injury: Proteomic Data Mining in Data Mining in Biomedicine. Pardalos P, Boginski V, Vazacopoulos A, Eds., Springer Press, New York, in press 2005.

-127. Ottens AK, Kobeissy FH, Haskins WE, Golden EC, Zhang ZQ, Chen S, Hayes RL, Wang KKW, Denslow ND. Neuroproteomics in Neurotrauma. Mass Spectrom Rev, (Invited Review), 2005 in press.

-128. Zhang Z, Ottens AK, Larner SF, Kobeissy FH, Williams ML, Hayes RL, Wang KKW. (2006) Direct Rho-associated kinase inhibition induces cofilin dephosphorylation and neurite outgrowth in PC-12 cells. Cell. Mol. Biol. Lett., 11:15-32. [PDF]

BOOK EDITED
Wang, K.K.W. and Yuen, P.-w. (Eds.) (1999) Calpain: The Pharmacology and Toxicology of Calcium-Dependent Protease. Taylor and Francis, PA; 22 Chapters, 441 pages [PDF]